Aldosterone inhibits nitric oxide synthesis in rat vascular smooth muscle cells induced by interleukin-1 beta

Eur J Pharmacol. 1995 Jul 18;290(2):69-73. doi: 10.1016/0922-4106(95)90018-7.

Abstract

We investigated the effects of aldosterone on nitric oxide (NO) synthesis in vascular smooth muscle cells. We measured the production of nitrite, a stable metabolite of NO, and the expression of inducible NO synthase mRNA and protein in cultured rat vascular smooth muscle cells. Incubation of the cultures with interleukin-1 beta (10 ng/ml) for 24 h caused a significant increase in nitrite generation. The interleukin-1 beta-induced nitrite production by vascular smooth muscle cells was significantly inhibited by aldosterone in a dose (10(-9) approximately 10(-6) M)-dependent manner. Incubation with interleukin-1 beta for 12 approximately 24 h caused inducible NO synthase mRNA expression in vascular smooth muscle cells, whereas aldosterone had a suppressive effect on its expression. Aldosterone also decreased interleukin-1 beta-induced NO synthase protein accumulation. These results indicate that aldosterone inhibits NO synthesis under interleukin-1 beta-stimulated conditions in vascular smooth muscle cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldosterone / pharmacology*
  • Analysis of Variance
  • Animals
  • Aorta, Thoracic
  • Blotting, Northern
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Humans
  • Immunoblotting
  • Interleukin-1 / pharmacology
  • Muscle, Smooth, Vascular / drug effects*
  • Muscle, Smooth, Vascular / metabolism
  • Nitric Oxide / biosynthesis*
  • Nitric Oxide Synthase / metabolism
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Time Factors

Substances

  • Interleukin-1
  • RNA, Messenger
  • Nitric Oxide
  • Aldosterone
  • Nitric Oxide Synthase