DNA adducts in urothelial cells: relationship with biomarkers of exposure to arylamines and polycyclic aromatic hydrocarbons from tobacco smoke

Int J Cancer. 1996 Jan 26;65(3):314-6. doi: 10.1002/(SICI)1097-0215(19960126)65:3<314::AID-IJC6>3.0.CO;2-2.


Markers of exposure to polycyclic aromatic hydrocarbons (urinary 1-hydroxypyrene-glucuronide) and aromatic amines (4-aminobiphenyl-hemoglobin adducts), as well as urinary mutagenicity, were measured in 47 healthy smokers and 50 nonsmokers. DNA adducts were determined by P32-postlabeling in the exfoliated bladder cells of 39 healthy subjects. Both 1-hydroxypyrene-glucuronide (1-OHPG) and 4-aminobiphenyl adducts (4-ABP-Hb) were associated with smoking habits, but only 4-ABP-Hb adducts were associated with consumption of black, air-cured tobacco. The levels of 2 DNA adducts (numbers 2 and 4) in urothelial cells were clearly associated with 4-ABP-Hb adducts, in all subjects and in smokers. Levels of one of these DNA adducts (number 2) were also associated with 1-hydroxypyrene-glucuronide in urines, but in smokers the association was not statistically significant. Overall, these observations constitute further evidence of a role of arylamines in tobacco-induced bladder cancer.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aminobiphenyl Compounds / adverse effects*
  • Cotinine / urine
  • DNA Adducts / biosynthesis*
  • DNA Adducts / urine
  • Humans
  • Male
  • Middle Aged
  • Nicotine / urine
  • Pyrenes / adverse effects*
  • Smoking / adverse effects
  • Urinary Bladder / cytology
  • Urinary Bladder / drug effects*
  • Urinary Bladder / metabolism


  • Aminobiphenyl Compounds
  • DNA Adducts
  • Pyrenes
  • Nicotine
  • pyrene
  • Cotinine