Transcriptional regulation of the yeast DnaJ homologue SIS1

J Biol Chem. 1996 Jan 19;271(3):1349-56. doi: 10.1074/jbc.271.3.1349.

Abstract

The Saccharomyces cerevisiae SIS1 gene encodes an essential heat shock protein with similarity to the Escherichia coli DnaJ protein. In sis 1-85 and sis1-86 mutants, the sis1 RNA is induced to high levels at room temperature and without heat shock. The presence of wild type SIS1 in the sis1-85 mutant represses the overexpression of SIS1-85 protein. Furthermore, overexpression of wild type SIS1 reduces the beta-galactosidase activity expressed from a SIS1:lacZ fusion. These results suggest that SIS1 negatively regulates its own expression. The autoregulation of SIS1 transcription is mediated through a 39-base pair cis-element containing the SIS1 heat shock element plus additional flanking sequences on one side. Although SIS1 transcription is constitutive, it is transiently induced upon heat shock. In addition, SIS1 transcription is regulated by SSA (a class of HSP70 proteins) function. The elevated transcription of SIS1 in ssa1 ssa2 mutants is mediated solely through the SIS1 heat shock element. Therefore, the SIS1 autoregulatory element is different from the SSA-responsive element, suggesting that the mechanism involved in autoregulation of SIS1 is distinct from regulation of SIS1 by SSA proteins.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • Escherichia coli / metabolism
  • Escherichia coli Proteins
  • Fungal Proteins / biosynthesis*
  • Fungal Proteins / genetics
  • Gene Expression Regulation, Fungal*
  • Genes, Fungal
  • HSP40 Heat-Shock Proteins
  • Heat-Shock Proteins / biosynthesis
  • Heat-Shock Proteins / genetics
  • Hot Temperature
  • Molecular Sequence Data
  • Plasmids
  • Promoter Regions, Genetic*
  • RNA, Fungal / biosynthesis
  • RNA, Messenger / biosynthesis
  • Recombinant Fusion Proteins / biosynthesis
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • Transcription, Genetic*
  • beta-Galactosidase / biosynthesis

Substances

  • DnaJ protein, E coli
  • Escherichia coli Proteins
  • Fungal Proteins
  • HSP40 Heat-Shock Proteins
  • Heat-Shock Proteins
  • RNA, Fungal
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • SIS1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • beta-Galactosidase