Comparison of conventional autoradiography with a new DNA enzyme immunoassay for the detection of hepatitis C virus-polymerase chain reaction amplification products

J Virol Methods. 1995 Sep;55(1):105-10. doi: 10.1016/0166-0934(95)00049-z.

Abstract

The detection of HCV-PCR amplification products by DNA enzyme immunoassay (DEIA) was compared with conventional hybridization carried out with a 32P-labelled oligonucleotide probe. The detection limit of both methods was shown to be between 100 pg and 1 ng of amplicon. All serum samples of 40 HCV-seropositive patients were positive after PCR in autoradiography, but only 38 with the DEIA technique (sensitivity 95%). There were no false-positive reactions by either method. The advantage of the DEIA method was the fast and non-radioactive detection of HCV amplicons. DEIA combines the specificity of the hybridization event with the speed of an ELISA procedure and is suitable for HCV-PCR.

Publication types

  • Comparative Study

MeSH terms

  • Autoradiography* / methods
  • Base Sequence
  • Chronic Disease
  • DNA Primers
  • DNA, Viral / analysis*
  • Hepacivirus / genetics*
  • Hepacivirus / isolation & purification
  • Hepatitis C / blood
  • Hepatitis C / virology
  • Humans
  • Immunoenzyme Techniques*
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*

Substances

  • DNA Primers
  • DNA, Viral