Abstract
The heat shock protein Hsp90 is known as an essential component of several signal transduction pathways and has now been identified as an essential host factor for hepatitis B virus replication. Hsp90 interacts with the viral reverse transcriptase to facilitate the formation of a ribonucleoprotein (RNP) complex between the polymerase and an RNA ligand. This RNP complex is required early in replication for viral assembly and initiation of DNA synthesis through a protein-priming mechanism. These results thus invoke a role for the Hsp90 pathway in the formation of an RNP.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Benzoquinones
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Carcinoma, Hepatocellular
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Cell Line
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Chickens
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DNA Replication*
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Enzyme Inhibitors / pharmacology
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HSP90 Heat-Shock Proteins / isolation & purification
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HSP90 Heat-Shock Proteins / metabolism*
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Hepatitis B virus / enzymology*
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Hepatitis B virus / physiology
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Kinetics
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Lactams, Macrocyclic
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Liver Neoplasms
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Models, Genetic
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Quinones / pharmacology
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RNA, Viral / metabolism*
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RNA-Directed DNA Polymerase / biosynthesis
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RNA-Directed DNA Polymerase / isolation & purification
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RNA-Directed DNA Polymerase / metabolism*
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Ribonucleoproteins / isolation & purification
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Ribonucleoproteins / metabolism
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Signal Transduction
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Transfection
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Tumor Cells, Cultured
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Virus Replication
Substances
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Benzoquinones
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Enzyme Inhibitors
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HSP90 Heat-Shock Proteins
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Lactams, Macrocyclic
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Quinones
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RNA, Viral
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Recombinant Proteins
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Ribonucleoproteins
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RNA-Directed DNA Polymerase
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geldanamycin