Downregulation of the human heme oxygenase gene by glucocorticoids and identification of 56b regulatory elements

Biochem Biophys Res Commun. 1996 Jan 26;218(3):759-65. doi: 10.1006/bbrc.1996.0135.


Several human heme oxygenase-1 promoter-driven chloramphenicol acetyltransferase constructs were examined in order to analyze promoter activity of the heme oxygenase-1 gene in microvessel endothelial cells. Heme oxygenase promoter activity was up-regulated by interleukin-6. This induction was shown to be down-regulated by glucocorticoids. Chloramphenicol acetyltransferase assays revealed that the promoter region (56 base pair) between -180 and -120 was responsible for up-regulation by growth factors, as well as for glucocorticoid-directed down-regulation. The same DNA fragments was shown to bind nuclear factor(s) from endothelial cells treated with dexamethasone. Formation of DNA protein complexes peaked after a 6-hour treatment. The DNA fragment was found to contain a sequence recognized by the STAT 3/acute phase response factor.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acute-Phase Reaction
  • Animals
  • Base Sequence
  • Dexamethasone / pharmacology*
  • Down-Regulation
  • Gene Expression Regulation, Enzymologic / drug effects
  • Glucocorticoids / pharmacology*
  • Heme Oxygenase (Decyclizing) / genetics*
  • Humans
  • Interleukin-6 / pharmacology
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • RNA, Messenger / genetics
  • Rabbits
  • Transcription Factors / physiology*


  • Glucocorticoids
  • Interleukin-6
  • RNA, Messenger
  • Transcription Factors
  • Dexamethasone
  • Heme Oxygenase (Decyclizing)

Associated data

  • GENBANK/X14782