Improvement of Salmonella detection on motility enrichment media by ferrioxamine E-supplementation of pre-enrichment culture

Int J Food Microbiol. 1995 Oct;27(2-3):147-59. doi: 10.1016/0168-1605(94)00160-8.

Abstract

Supplementation of buffered peptone water with ferrioxamine E in concentrations ranging from 0.001 to 1.0 microgram/ml significantly increased the motility of Salmonella on MSRV and DIASSALM semi-solid enrichment media. Zone diameters of swarming S. enteritidis and S. typhimurium increased more than twofold following use of pre-enrichment cultures supplemented with 0.01 microgram ferrioxamine E/ml. The activity of ferrioxamine E is similar at 37 degrees C and 42 degrees C. Pre-enrichment of Salmonella in a variety of foods in supplemented buffered peptone water, with shaking at 37 degrees C for 6 h and motility enrichment at 42 degrees C for 16 h, enabled motile Salmonella to be detected in 1 day.

MeSH terms

  • Culture Media / chemistry*
  • Ferric Compounds*
  • Microbiological Techniques
  • Peptides, Cyclic*
  • Peptones
  • Salmonella / drug effects
  • Salmonella / isolation & purification*
  • Salmonella / physiology
  • Temperature

Substances

  • Culture Media
  • Ferric Compounds
  • Peptides, Cyclic
  • Peptones
  • ferrioxamine E