The inhibitory effect of astragalus membranaceus on coxsackie B-3 virus RNA replication

Chin Med Sci J. 1995 Sep;10(3):146-50.


Using mice infected with coxsackie B-3 virus (CVB3) as a viral myocarditis model, we observed the inhibitory effect of Astragalus membranaceus (AM) on CVB3-RNA replication in myocardial tissue of mice by RNA-RNA in situ hybridization with negative-strand RNA probes labelled with 35S and quantitative imaging analysis of positive signals. The mechanism of its effect on CVB3-RNA replication has been investigated by detection of beta-interferon (beta-IFN) as well. Results showed that the copy numbers of CVB3-RNA as well as the histologic scores (necrosis) in myocardial tissues of infected-AM treated mice were significantly lower than those in infected and normal saline treated mice, suggesting that AM could inhibit the replication of CVB3-RNA, but its effect on CVB3-RNA replication had no correlation with induction of beta-IFN.

MeSH terms

  • Animals
  • Antiviral Agents / pharmacology*
  • Astragalus propinquus
  • Coxsackievirus Infections / drug therapy
  • Drugs, Chinese Herbal / pharmacology*
  • Enterovirus B, Human / physiology*
  • Interferon-beta / analysis
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Myocarditis / drug therapy
  • Myocarditis / virology
  • RNA, Viral / drug effects
  • Virus Replication / drug effects*


  • Antiviral Agents
  • Drugs, Chinese Herbal
  • RNA, Viral
  • Interferon-beta