A new dominant selectable marker for genetic transformation; Hsp70-opd

Insect Biochem Mol Biol. 1995 Dec;25(10):1061-5. doi: 10.1016/0965-1748(95)00061-5.

Abstract

New P element plasmids containing the organophosphate-degrading gene opd as a dominant selectable marker were tested as transformation vectors in Drosophila melanogaster. One of these vectors was modified by the addition of the D. melanogaster mini-white gene as a comarker. When transformed individuals were identified using paraoxon selection for opd alone, results were similar to those obtained with mini-white. No false positives were recovered, however one strain contained the mini-white gene but inadequate resistance to survive our screening regimen due to a defective Hsp70-opd gene. Results suggest that Hsp70-opd is similar to mini-white for distinguishing transformed individuals but does not require time-consuming individual examination. Due to the mode of action of organophosphorus nerve agents, Hsp70-opd has potential as a selectable marker in numerous animals beside fruit flies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Drosophila melanogaster / genetics*
  • Genetic Markers*
  • HSP70 Heat-Shock Proteins / genetics*
  • Insecticide Resistance / genetics
  • Insecticides
  • Paraoxon
  • Phosphoric Monoester Hydrolases / genetics
  • Phosphoric Monoester Hydrolases / metabolism
  • Plasmids
  • Transformation, Genetic*

Substances

  • Genetic Markers
  • HSP70 Heat-Shock Proteins
  • Insecticides
  • phosphorylphosphatase
  • Phosphoric Monoester Hydrolases
  • Paraoxon