Mitochondrial ribosomal protein L11 gene of Dictyostelium discoideum resides not in the nuclear genome but in the mitochondrial genome

DNA Res. 1995 Jun 30;2(3):129-32. doi: 10.1093/dnares/2.3.129.

Abstract

During the course of analysis of the mitochondrial genome of the cellular slime mold, Dictyostelium discoideum, we found a gene (rpl11) for mitochondrial ribosomal protein (RPL11), having 172 amino acid residues. Southern blot analysis revealed that the gene resided in the mitochondrial DNA as a single-copy but not in the nuclear DNA. From Northern blot experiments, one major mRNA (about 27 kb) and two minor mRNAs (about 4 and 5 kb) for the gene were detected in the mitochondria. This is the first report showing that the active gene for RPL11 still resides in the mitochondrial genome and has not been transferred to the nuclear genome in D. discoideum.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Cell Compartmentation*
  • Cell Nucleus / genetics
  • Dictyostelium / genetics*
  • Genes, Fungal*
  • Mitochondria / genetics*
  • Molecular Sequence Data
  • RNA, Messenger / analysis
  • Ribosomal Proteins / genetics*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • RNA, Messenger
  • Ribosomal Proteins
  • ribosomal protein L11

Associated data

  • GENBANK/D21196