A high-performance liquid chromatographic assay for the determination of itraconazole concentration using solid-phase extraction and small sample volume

Ther Drug Monit. 1995 Oct;17(5):522-5. doi: 10.1097/00007691-199510000-00014.


Itraconazole has been shown to be highly effective against a broad spectrum of fungi, particularly aspergillus. In order to achieve the desired pharmacological effect, the itraconazole plasma concentration should be > 250 ng/ml. We developed a high-performance liquid chromatography assay for the rapid determination of plasma itraconazole concentration using solid-phase extraction and small sample volume. The assay possessed linearity up to 5,000 ng/ml, sensitivity of 50 ng/ml, average recovery of 100.8%, and run-to-run precision (n = 10) for concentrations of 100, 200, and 500 ng/ml of 12, 6.6, and 5.8%, respectively. Furthermore, the assay proved to be free of interference from 48 commonly prescribed and some over-the-counter medications. We conclude that the method described here is ideally suited for the therapeutic monitoring of itraconazole.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetonitriles / chemistry
  • Antifungal Agents / blood*
  • Chromatography, High Pressure Liquid / methods*
  • Drug Interactions
  • Drug Monitoring
  • Humans
  • Itraconazole / blood*
  • Regression Analysis


  • Acetonitriles
  • Antifungal Agents
  • Itraconazole
  • acetonitrile