An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies

Electrophoresis. 1995 Oct;16(10):1977-81. doi: 10.1002/elps.11501601324.


We have improved upon the reference two-dimensional (2-D) electrophoretic map of rat liver proteins originally published in 1991 (N. L. Anderson et al., Electrophoresis 1991, 12, 907-930). A total of 53 proteins (102 spots) are now identified, many by microsequencing. In most cases, spots cut from wet, Coomassie Blue stained 2-D gels were submitted to internal tryptic digestion [2], and individual peptides, separated by high-performance liquid chromatography (HPLC), were sequenced using a Perkin-Elmer 477A sequenator. Additional spots were identified using specific antibodies.

MeSH terms

  • Animals
  • Chromatography, High Pressure Liquid
  • Electrophoresis, Gel, Two-Dimensional*
  • Hydrogen-Ion Concentration
  • Information Systems*
  • Isoelectric Point
  • Liver / chemistry*
  • Mice
  • Molecular Weight
  • Peptide Fragments / chemistry
  • Proteins / analysis
  • Proteins / chemistry*
  • Rats
  • Sequence Analysis
  • Trypsin


  • Peptide Fragments
  • Proteins
  • Trypsin