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, 85, 303-7

Analysis of Glucan Synthesis by Streptococcus Mutans

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  • PMID: 8586194

Analysis of Glucan Synthesis by Streptococcus Mutans

H K Kuramitsu et al. Dev Biol Stand.

Abstract

Glucan synthesis by Streptococcus mutans GS-5 has been analyzed by examining the regulation of expression and structure-function relationships of the glucosyltransferases. Primer extension analysis of the gtf genes has identified the putative promoter sequences for these genes and indicated that the -10 sequences are similar to the Escherichia coli consensus sequence. Site-directed mutagenesis as well as deletion analysis of the enzymes have identified amino acid residues as well as functional domains which play important roles in glucan synthesis.

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