Effect of hypergastrinemia and blockade of gastrin-receptors on pancreatic growth in the mouse

Hepatogastroenterology. Jul-Aug 1995;42(4):423-31.

Abstract

Background/aim: The present study evaluates whether endogenous gastrin regulates pancreatic growth in the mouse.

Materials and methods: Male NMRI mice weighing 12-15 g were assigned to six groups (10 mice/group) which were treated with different combinations of 0.9% NaCl, omeprazole, a CCK-A antagonist, a CCK-B antagonist, loxiglumide, and L365, 260 for 10 days each according to different protocols.

Results: Omeprazole caused a marked, 10-fold increase in serum gastrin which was not affected by the gastrin antagonist, but markedly reduced by the CCK-A antagonist. The marked increase in endogenous gastrin caused by omeprazole did not promote pancreatic growth in any way. Similarly, the gastrin antagonist did not inhibit pancreatic growth. In contrast, the CCK-A antagonist significantly decreased pancreatic weight and protein content.

Conclusions: The present results strongly suggest that endogenous gastrin--in contrast to CCK--does not regulate pancreatic growth in the mouse. The inhibitory effect of loxiglumide on omeprazole-induced increase in serum gastrin might be explained by recent findings which showed that CCK-A antagonists can stimulate gastric acid secretion probably due to a reduction of the inhibitory effect of basal CCK on the D-cell and its somatostatin release. Probably such a slight stimulation of gastric acid secretion caused by the CCK-A antagonist might reduce the gastrin increase caused by omeprazole's abolishment of acid secretion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Enzyme Inhibitors / pharmacology
  • Gastrins / blood
  • Gastrins / physiology*
  • Hormone Antagonists / pharmacology
  • Male
  • Mice
  • Mice, Inbred Strains
  • Omeprazole / pharmacology
  • Pancreas / drug effects
  • Pancreas / growth & development*
  • Proglumide / analogs & derivatives
  • Proglumide / pharmacology
  • Receptors, Cholecystokinin / antagonists & inhibitors*

Substances

  • Enzyme Inhibitors
  • Gastrins
  • Hormone Antagonists
  • Receptors, Cholecystokinin
  • loxiglumide
  • Proglumide
  • Omeprazole