The human TAX1 gene encoding the axon-associated cell adhesion molecule TAG-1/axonin-1: genomic structure and basic promoter

Genomics. 1995 Nov 20;30(2):141-8. doi: 10.1006/geno.1995.9892.

Abstract

The human TAX-1 gene (HGMW-approved symbol TAX1) is located on chromosome 1 (1q32.1) and encodes the neuronal cell adhesion molecule TAG-1/axonin-1. The gene product, termed TAG-1 in the rat and axonin-1 in the chicken, is composed of six immunoglobulin (Ig)-like and four fibronectin type III (FNIII)-like domains. It is found predominantly on the axons of particular nerve fiber tracts during neural development, and it has been demonstrated to function as a potent substratum for neurite outgrowth in vitro. Here we report the cloning and structural characterization of the TAX-1 gene. The transcribed region of the TAX-1 gene extends over about 40 kb. Like its chicken homologue, the human TAX-1 gene consists of 23 exons. Two GT/CA microsatellites were localized in the first intron; a polymorphism was found for one of them. Reporter gene analysis with serially truncated fragments of the 5'-flanking region indicated that a 164-bp fragment located immediately upstream of the putative transcription initiation site was sufficient to function as a basal promoter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Adhesion Molecules, Neuronal / genetics*
  • Chickens
  • Chromosome Mapping
  • Cloning, Molecular
  • Contactin 2
  • Exons
  • Genes, Reporter
  • Humans
  • Introns
  • Molecular Sequence Data
  • Promoter Regions, Genetic*
  • Repetitive Sequences, Nucleic Acid
  • Sequence Homology, Nucleic Acid

Substances

  • CNTN2 protein, human
  • Cell Adhesion Molecules, Neuronal
  • Cntn2 protein, rat
  • Contactin 2

Associated data

  • GENBANK/X84419
  • GENBANK/X84420
  • GENBANK/X85978