Recovery of mycobacteria from blood in mycobacteria growth indicator tube and Lowenstein-Jensen slant after lysis-centrifugation

J Clin Microbiol. 1995 Dec;33(12):3315-6. doi: 10.1128/jcm.33.12.3315-3316.1995.

Abstract

Five hundred five blood samples for culture were processed in the Isolator lysis-centrifugation system and were then inoculated into a Mycobacteria Growth Indicator Tube (MGIT) and onto a Lowenstein-Jensen (L-J) slant. Forty-nine isolates of Mycobacterium avium complex and three isolates of Mycobacterium tuberculosis were recovered from 50 of the blood culture specimens. Forty-five isolates from 43 specimens were recovered in the MGIT, with a mean time to detection of 21 days. Forty-one isolates from 40 specimens were recovered in the L-J slants, and the mean time to detection was 36 days. Nine specimens were positive in the MGIT alone, while seven specimens were positive only in L-J medium.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • AIDS-Related Opportunistic Infections / complications
  • AIDS-Related Opportunistic Infections / diagnosis
  • AIDS-Related Opportunistic Infections / microbiology
  • Bacteriological Techniques*
  • Blood / microbiology*
  • Centrifugation
  • Culture Media
  • Evaluation Studies as Topic
  • Hemolysis
  • Humans
  • Mycobacterium avium Complex / growth & development
  • Mycobacterium avium Complex / isolation & purification*
  • Mycobacterium avium-intracellulare Infection / complications
  • Mycobacterium avium-intracellulare Infection / diagnosis
  • Mycobacterium avium-intracellulare Infection / microbiology
  • Mycobacterium tuberculosis / growth & development
  • Mycobacterium tuberculosis / isolation & purification*
  • Time Factors
  • Tuberculosis, Pulmonary / complications
  • Tuberculosis, Pulmonary / diagnosis
  • Tuberculosis, Pulmonary / microbiology

Substances

  • Culture Media