Rhamnogalacturonase B from Aspergillus aculeatus is a rhamnogalacturonan alpha-L-rhamnopyranosyl-(1-->4)-alpha-D-galactopyranosyluronide lyase

Plant Physiol. 1996 Jan;110(1):73-7. doi: 10.1104/pp.110.1.73.

Abstract

The recently described rhamnogalacturonase B, which is able to degrade ramified hairy regions of pectin, was found to be a rhamnogalacturonan alpha-L-rhamnopyranosyl-(1-->4)-alpha-D-galactopyranosyluronide lyase. The cleavage site and mechanism differ from that of the previously described rhamnogalacturonase A, which is a hydrolase and can now be termed rhamnogalacturonan alpha-D-galactopyranosyluronide-(1-->2)-alpha-L-rhamnopyranosyl hydrolase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus / enzymology*
  • Carbohydrate Sequence
  • Chromatography, High Pressure Liquid
  • Enzymes / classification
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Oligosaccharides / metabolism
  • Polysaccharide-Lyases / genetics
  • Polysaccharide-Lyases / metabolism*
  • Recombinant Proteins / metabolism
  • Substrate Specificity

Substances

  • Enzymes
  • Oligosaccharides
  • Recombinant Proteins
  • Polysaccharide-Lyases
  • rhamnogalacturonase B