Identification of a new Arabidopsis disease resistance locus, RPs4, and cloning of the corresponding avirulence gene, avrRps4, from Pseudomonas syringae pv. pisi

Mol Plant Microbe Interact. 1996 Jan;9(1):55-61. doi: 10.1094/mpmi-9-0055.

Abstract

A molecular genetic approach was used to study the interaction between Arabidopsis and an avirulence gene from Pseudomonas syringae pv. pisi. P. syringae pv. pisi strain 151 induces a hypersensitive response (HR) when inoculated on the Arabidopsis accession Po-1. A genomic cosmid library was constructed from DNA from P. syringae pv. pisi strain 151 and a cosmid was identified that causes the normally virulent P. syringae pv. tomato strain DC3000 to induce an HR on Po-1. The cosmid was subcloned and a 1.2-kb DNA fragment conferring avirulence activity was sequenced. The single significant open reading frame within the 1.2-kb fragment was designated avrRps4. An avrRps4-specific probe hybridizes to DNA from all P. syringae pv. pisi strains tested. P. syringae strains carrying avrRps4 induces an HR on specific accessions of both Arabidopsis and soybean. Arabidopsis accession Ws-0 is resistant to DC3000(avrRps4), whereas accession RLD is susceptible. Resistance segregates as a single dominant locus in a genetic cross between Ws-0 and RLD. This disease resistance locus, RPS4, was mapped to chromosome 5 between the molecular markers sAT2105 and KG-8.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Arabidopsis / microbiology
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • Crosses, Genetic
  • Genes, Bacterial*
  • Genes, Plant*
  • Genetic Variation
  • Genomic Library
  • Molecular Sequence Data
  • Open Reading Frames
  • Plant Diseases / genetics*
  • Plant Proteins / genetics
  • Pseudomonas / classification
  • Pseudomonas / genetics*
  • Pseudomonas / pathogenicity
  • Sequence Analysis, DNA
  • Soybeans / microbiology
  • Species Specificity

Substances

  • AvrRps4 protein, Pseudomonas syringae
  • Bacterial Proteins
  • Plant Proteins
  • rps4 protein, plant

Associated data

  • GENBANK/L43559