Isolation and characterization of a pSym locus of Rhizobium sp. BR816 that extends nodulation ability of narrow host range Phaseolus vulgaris symbionts to Leucaena leucocephala

Mol Plant Microbe Interact. 1996 Jan;9(1):74-7. doi: 10.1094/mpmi-9-0074.

Abstract

Introduction of a cosmid library of megaplasmid DNA of Rhizobium sp. BR816, a broad host range Rhizobium strain, into R. etli CE3, a narrow host range bean symbiont, resulted in the isolation of a transconjugant that could effectively nodulate Leucaena leucocephala. Analysis of the corresponding cosmid, pBRF2, revealed the presence of genes required for elicting nitrogen-fixing nodules on L. leucoephala. Subcloning and Tn5 tagging identified a locus responsible for the host range extension. Sequence analysis of this locus revealed an ORF that shows significant identity with NodO of R. leguminosarum bv. viciae.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Calcium-Binding Proteins*
  • Fabaceae / microbiology*
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Phenotype
  • Plants, Medicinal*
  • Rhizobium / genetics*
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Symbiosis / genetics*

Substances

  • Bacterial Proteins
  • Calcium-Binding Proteins
  • NodO protein, Leucaena leucocephala
  • NodO protein, Rhizobium leguminosarum

Associated data

  • GENBANK/U26451