Immunohistochemical expression of glutathione S-transferase pi (GST-pi) and chemotherapy response in malignant ovarian tumors

J Obstet Gynaecol (Tokyo 1995). 1995 Jun;21(3):305-12. doi: 10.1111/j.1447-0756.1995.tb01015.x.

Abstract

Objectives: To clarify the relationship between the expression of GST-pi and the therapeutic effects of high-dose combination chemotherapy with support by autologous bone-marrow transplantation (ABMT).

Methods: Expression of GST-pi in tissue specimen were analyzed by indirect immunoperoxidase methods using anti-GST-pi polyclonal antibody.

Results: (1) Eighteen of 36 cases (50%) tested prior to administration of any chemotherapeutic agents were found to be positive in terms of immunohistochemical expression of GST-pi in the cancer cells. (2) The rate of GST-pi expression was elevated following administration of cisplatin, adriamycin, and cyclophosphamide, which suggests that administration of such anti-cancer agents is capable of inducing expression of GST-pi in cancer cells. (3) GST-pi negative patients exhibit better survival rates than do GST-pi positive patients (Kaplan Meier method: p < 0.001).

Conclusions: Analysis of GST-pi obtained at primary surgery can serve as a valid marker in anticipating chemotherapeutic effects and long-term prognoses.

MeSH terms

  • Antineoplastic Combined Chemotherapy Protocols / therapeutic use
  • Female
  • Glutathione Transferase / metabolism*
  • Humans
  • Immunoenzyme Techniques
  • Ovarian Neoplasms / drug therapy*
  • Ovarian Neoplasms / enzymology*
  • Ovarian Neoplasms / mortality
  • Ovarian Neoplasms / pathology
  • Prognosis
  • Survival Analysis
  • Treatment Outcome

Substances

  • Glutathione Transferase