The expression of a specific 2-deoxyglucose-6P phosphatase prevents catabolite repression mediated by 2-deoxyglucose in yeast

Curr Genet. 1995 Jul;28(2):101-7. doi: 10.1007/BF00315774.

Abstract

2-deoxyglucose (2-DOG), a non-metabolize analogue of glucose, is taken up by yeast using the same transporter(s) as glucose and is phosphorylated by hexokinases producing 2-deoxyglucose-6-P. We found that in DOGR yeasts, 2-DOG was not able to trigger glucose repression, even at concentrations of 0.5%. This result suggests that the specific 2-DOG-6P phosphatase, the enzyme responsible for the DOGR phenotype, may be involved in inhibiting the process of catabolite repression mediated by 2-DOG.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Deoxyglucose / genetics
  • Deoxyglucose / metabolism*
  • Genes, Fungal
  • Kinetics
  • Phosphoric Monoester Hydrolases / metabolism*
  • Repressor Proteins / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • Transformation, Genetic

Substances

  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Deoxyglucose
  • DOG2 protein, S cerevisiae
  • Phosphoric Monoester Hydrolases