Release of ferulic acid from wheat bran by a ferulic acid esterase (FAE-III) from Aspergillus niger

Appl Microbiol Biotechnol. 1995 Nov;43(6):1082-7. doi: 10.1007/BF00166929.

Abstract

Ferulic acid was efficiently released from a wheat bran preparation by a ferulic acid esterase from Aspergillus niger (FAE-III) when incubated together with a Trichoderma viride xylanase (a maximum of 95% total ferulic acid released after 5 h incubation). FAE-III by itself could release ferulic acid but at a level almost 24-fold lower than that obtained in the presence of the xylanase (2 U). Release of ferulic acid was proportional to the FAE-III concentration between 0.1 U and 1.3 U, but the presence of low levels of xylanase (0.1 U) increased the amount of ferulic acid released 6-fold. Total sugar release was not influenced by the action of FAE-III on the wheat bran, but the rate of release of the apparent end-products of xylanase action (xylose and xylobiose) was elevated by the presence of the esterase. The results show that FAE-III and the xylanase act together to break down feruloylated plant cell-wall polysaccharides to give a high yield of ferulic acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus niger / enzymology*
  • Carboxylic Ester Hydrolases / metabolism*
  • Cell Wall / metabolism*
  • Coumaric Acids / metabolism*
  • Dietary Fiber / metabolism*
  • Disaccharides / isolation & purification
  • Fungal Proteins / metabolism*
  • Molecular Weight
  • Trichoderma / enzymology
  • Xylan Endo-1,3-beta-Xylosidase
  • Xylose / isolation & purification
  • Xylosidases / metabolism

Substances

  • Coumaric Acids
  • Dietary Fiber
  • Disaccharides
  • Fungal Proteins
  • Xylose
  • ferulic acid
  • Carboxylic Ester Hydrolases
  • feruloyl esterase
  • Xylosidases
  • Xylan Endo-1,3-beta-Xylosidase
  • xylobiose