Stimulatory effects of fibronectin and EGF on migration of corneal epithelial cells

Invest Ophthalmol Vis Sci. 1987 Feb;28(2):205-11.


The effects of fibronectin and epidermal growth factor (EGF) on the migration of corneal epithelial cells in situ were investigated. When rabbit corneal blocks were cultured in serum-free medium with fibronectin or EGF, corneal epithelial cells migrated on the cut surface of the stroma. Histological and electron microscopic studies revealed that the leading edge of the fibronectin-treated cornea was thin, single-layered epithelium, whereas the leading edge of the EGF-treated cornea was a hypertrophic, multilayered epithelium. By autoradiography with 3H-thymidine, the authors observed that EGF stimulated corneal epithelial cell proliferation; however, fibronectin had no effect on cell proliferation. These results demonstrate that fibronectin and EGF enhance migration of corneal epithelial cells in different ways: Fibronectin facilitates sliding of epithelium, whereas EGF increases cell proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Division / drug effects
  • Cell Movement / drug effects
  • Cornea / cytology
  • Cornea / drug effects
  • Cornea / physiology*
  • Culture Media, Serum-Free
  • Epidermal Growth Factor / pharmacology*
  • Epithelial Cells
  • Epithelium / drug effects
  • Epithelium / physiology
  • Fibronectins / isolation & purification
  • Fibronectins / pharmacology*
  • Microscopy, Electron, Scanning
  • Organ Culture Techniques
  • Rabbits


  • Culture Media, Serum-Free
  • Fibronectins
  • Epidermal Growth Factor