Protein kinase C beta 1 overexpression augments phorbol ester-induced increase in endothelial permeability

J Cell Physiol. 1996 Feb;166(2):249-55. doi: 10.1002/(SICI)1097-4652(199602)166:2<249::AID-JCP2>3.0.CO;2-P.


We studied the postulated involvement of the protein kinase C beta 1 (PKC beta 1) isoform in the regulation of endothelial permeability using human dermal microvascular endothelial cell line (HMEC-1). We overexpressed the recombinant PKC beta 1 gene via retroviral-mediated transduction in these cells. PKC beta 1 gene transfer was stable, and PKC beta 1 protein production was persistent for at least 1 month posttransduction. Addition of 2 x 10(-9) M and 2 x 10(-8) M phorbol 12-myristate 13-acetate (PMA) to the control (nontransduced) HMEC-1 cells increased the transendothelial 125I-albumin clearance rate (an index of endothelial permeability) from 2.5 +/- 0.2 x 10(-2) microliters/min to 5.4 +/- 1.2 x 10(-2) microliters/min and 16.8 +/- 3.1 x 10(-2) microliters/min, respectively. However, addition of 2 x 10(-9) M PMA to PKC beta 1-overexpressing HMEC-1 cells produced a maximal increase in the transendothelial 125I-albumin clearance rate of 15.9 +/- 2.0 x 10(-2) microliters/min. Challenge of these cells with 2 x 10(-8) M PMA did not further augment the increase in permeability. Activation with PMA was associated with the translocation of the PKC beta 1 from the cytosol to the membrane. These data show that PKC beta 1 overexpression augments the increase in endothelial permeability in response to PKC activation, suggesting an important function for the PKC beta 1 isoform in the regulation of endothelial barrier.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Capillary Permeability / drug effects*
  • Cell Line
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / metabolism*
  • Humans
  • Protein Kinase C / genetics
  • Protein Kinase C / metabolism*
  • Protein Kinase C beta
  • Recombinant Proteins
  • Retroviridae / physiology
  • Serum Albumin / pharmacokinetics
  • Tetradecanoylphorbol Acetate / pharmacology*
  • Transfection


  • Recombinant Proteins
  • Serum Albumin
  • Protein Kinase C
  • Protein Kinase C beta
  • Tetradecanoylphorbol Acetate