Extracellular dopamine, norepinephrine, and serotonin in the nucleus accumbens of freely moving rats during intracerebral dialysis with cocaine and other monoamine uptake blockers

J Neurochem. 1996 Feb;66(2):559-68. doi: 10.1046/j.1471-4159.1996.66020559.x.


Monoamine-uptake blockers were applied focally (0.1-1,000 microM) through a dialysis probe in the nucleus accumbens of freely moving rats, and the extracellular concentrations of dopamine, norepinephrine, and serotonin were measured. The selective dopamine-uptake blocker GBR 12935 increased dopamine preferentially with only a small effect on norepinephrine, whereas the selective serotonin-uptake blocker fluoxetine increased serotonin output preferentially. In contrast, the selective norepinephrine-uptake blockers desipramine and nisoxetine enhanced not only norepinephrine, but also serotonin and dopamine appreciably. Cocaine increased all three amines with the greatest effects on dopamine and serotonin. As in our previous study on the ventral tegmental area, there was a positive association between dopamine and norepinephrine output when all blocker data were taken together. The present results suggest a contribution of the increase in norepinephrine, but not serotonin, to the enhancement of dopamine after cocaine applied focally in the nucleus accumbens.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biogenic Monoamines / antagonists & inhibitors
  • Biogenic Monoamines / metabolism
  • Cocaine / administration & dosage
  • Cocaine / pharmacology*
  • Dialysis
  • Dopamine / metabolism*
  • Extracellular Space / metabolism
  • Neurotransmitter Uptake Inhibitors / administration & dosage
  • Neurotransmitter Uptake Inhibitors / pharmacology*
  • Norepinephrine / metabolism*
  • Nucleus Accumbens / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Serotonin / metabolism*


  • Biogenic Monoamines
  • Neurotransmitter Uptake Inhibitors
  • Serotonin
  • Cocaine
  • Dopamine
  • Norepinephrine