Desulfitobacterium sp. strain PCE1, an anaerobic bacterium that can grow by reductive dechlorination of tetrachloroethene or ortho-chlorinated phenols

Arch Microbiol. 1996 Feb;165(2):132-40. doi: 10.1007/s002030050308.

Abstract

A strictly anaerobic bacterium, strain PCE1, was isolated from a tetrachloroethene-dechlorinating enrichment culture. Cells of the bacterium were motile curved rods, with approximately four lateral flagella. They possessed a gram-positive type of cell wall and contained cytochrome c. Optimum growth occurred at pH 7.2-7.8 and 34-38 degrees C. The organism grew with L-lactate, pyruvate, butyrate, formate, succinate, or ethanol as electron donors, using either tetrachloroethene, 2-chlorophenol, 2,4,6-trichlorophenol, 3-chloro-4-hydroxy-phenylacetate, sulfite, thiosulfate, or fumarate as electron acceptors. Strain PCE1 also grew fermentatively with pyruvate as the sole substrate. L-Lactate and pyruvate were oxidized to acetate. Tetrachloroethene was reductively dechlorinated to trichloroethene and small amounts ( 5%) of cis-1,2-dichloroethene and trans-1,2-dichloroethene. Chlorinated phenolic compounds were dechlorinated specifically at the ortho-position. On the basis of 16S rRNA sequence analysis, the organism was identified as a species within the genus Desulfitobacterium, which until now only contained the chlorophenol-dechlorinating bacterium, Desulfitobacterium dehalogenans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria, Anaerobic / cytology
  • Bacteria, Anaerobic / growth & development
  • Bacteria, Anaerobic / isolation & purification
  • Bacteria, Anaerobic / metabolism*
  • Biodegradation, Environmental
  • Chlorophenols / metabolism*
  • Culture Media
  • Hydrogen-Ion Concentration
  • Lactates / metabolism
  • Lactic Acid
  • Molecular Sequence Data
  • Oxidation-Reduction
  • Pyruvates / metabolism
  • Pyruvic Acid
  • Soil Microbiology*
  • Temperature
  • Tetrachloroethylene / metabolism*

Substances

  • Chlorophenols
  • Culture Media
  • Lactates
  • Pyruvates
  • Lactic Acid
  • Pyruvic Acid
  • Tetrachloroethylene

Associated data

  • GENBANK/X81032