Structure of the O-specific Polysaccharide Chain of Shigella Boydii Type 5 Lipopolysaccharide: A Repeated Study

Carbohydr Res. 1995 Dec 27;279:183-92. doi: 10.1016/0008-6215(95)00276-6.

Abstract

An acidic, partially O-acetylated O-specific polysaccharide was obtained by mild acid degradation of Shigella boydii type 5 lipopolysaccharide and studied by 1H and 13C NMR spectroscopy, including 2D COSY, 13C-1H heteronuclear COSY, 1D NOE, and 2D ROESY experiments, and chemical methods (sugar and methylation analysis, O-deacetylation, carboxyl reduction, solvolysis with anhydrous HF, partial acid hydrolysis. Smith degradation). It was concluded that the polysaccharide has a hexasaccharide repeating unit of the following structure: [Formula: See Text] with the degree of O-acetylation varying over 30-50%. The established structure differs from that proposed recently for the O-specific polysaccharide of the same S. boydii serotype [M.J. Albert et al., Carbohydr. Res., 265 (1994) 121-127].

MeSH terms

  • Acetylation
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • O Antigens / chemistry*
  • Shigella boydii / chemistry*

Substances

  • O Antigens