Identification and characterization of CEN12 in the budding yeast Saccharomyces cerevisiae

Curr Genet. 1995 Nov;28(6):512-6. doi: 10.1007/BF00518162.


In this paper we report the cloning, sequencing and functional characterization of CEN12 and an associated autonomously replicating sequence (ARS) from the budding yeast Saccharomyces cerevisiae. In the course of studying a dynamin-related gene, DNM1, we previously physically mapped the gene to chromosome 12. Genetic mapping showed that the gene was tightly linked (0.35 cM) to the centromere. Subcloning experiments revealed that a centromere-like activity was included in a small segment of DNA immediately downstream from the DNM1 gene. Mitotic centromere activity was discerned by the ability of the region to de-stabilize a centromere-containing plasmid, and to stabilize an ARS-containing plasmid. Meiotic centromere activity was determined by the first-division segregation in crosses of ARS plasmids containing this region. The DNA sequence of this region revealed a sequence with strong homology to the consensus for yeast centromeres.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Centromere / genetics*
  • Chromosome Mapping
  • Chromosomes, Fungal
  • Cloning, Molecular
  • DNA Replication
  • Fungal Proteins / genetics*
  • GTP Phosphohydrolases*
  • Mitochondrial Proteins
  • Molecular Sequence Data
  • Plasmids
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*


  • Fungal Proteins
  • Mitochondrial Proteins
  • Saccharomyces cerevisiae Proteins
  • GTP Phosphohydrolases
  • DNM1 protein, S cerevisiae

Associated data

  • GENBANK/L40588