Induction of macrophage apoptosis by Bordetella pertussis adenylate cyclase-hemolysin

FEMS Microbiol Lett. 1995 Dec 1;134(1):27-32. doi: 10.1111/j.1574-6968.1995.tb07909.x.

Abstract

We previously demonstrated that Bordetella pertussis induced macrophage apoptosis and that adenylate cyclase-hemolysin expression is required for this phenomenon. In order to determine a role for other B. pertussis factors, such as pertussis toxin or the adhesins, pertactin and filamentous hemagglutinin, we compared mutants deficient in adhesin expression or purified proteins for their ability to induce cell death. We showed that only purified adenylate cyclase-hemolysin, but neither adhesins, nor pertussis toxin, killed macrophages through apoptosis. We also demonstrated that all activities of adenylate cyclase-hemolysin were required. Moreover, our results indicate that B. pertussis does not have to invade cells to be lethal.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylate Cyclase Toxin
  • Adenylyl Cyclases / metabolism
  • Adhesins, Bacterial / pharmacology
  • Animals
  • Apoptosis*
  • Bacterial Outer Membrane Proteins / pharmacology
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Bacterial Proteins / pharmacology*
  • Bordetella pertussis / chemistry*
  • DNA / metabolism
  • Hemagglutinins / pharmacology
  • Hemolysin Proteins / pharmacology*
  • Macrophages / cytology*
  • Mice
  • Pertussis Toxin
  • Protein Precursors / genetics
  • Protein Precursors / metabolism
  • Protein Precursors / pharmacology*
  • Recombinant Proteins / pharmacology
  • Virulence Factors, Bordetella / pharmacology

Substances

  • Adenylate Cyclase Toxin
  • Adhesins, Bacterial
  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Hemagglutinins
  • Hemolysin Proteins
  • Protein Precursors
  • Recombinant Proteins
  • Virulence Factors, Bordetella
  • pertactin
  • DNA
  • Pertussis Toxin
  • Adenylyl Cyclases