Purification of DNA polymerase-primase (DNA polymerase alpha) and DNA polymerase delta from embryos of Drosophila melanogaster

Methods Enzymol. 1995;262:62-77. doi: 10.1016/0076-6879(95)62009-5.
No abstract available

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Centrifugation, Density Gradient / methods
  • Chromatography / methods
  • Chromatography, Affinity / methods
  • Chromatography, Gel / methods
  • Chromatography, Ion Exchange / methods
  • DNA Polymerase II / analysis
  • DNA Polymerase II / isolation & purification*
  • DNA Polymerase II / metabolism
  • DNA Polymerase III
  • DNA Primase
  • DNA-Directed DNA Polymerase / analysis
  • DNA-Directed DNA Polymerase / isolation & purification*
  • DNA-Directed DNA Polymerase / metabolism
  • Deoxyadenine Nucleotides / metabolism
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / enzymology*
  • Durapatite
  • Electrophoresis, Polyacrylamide Gel / methods
  • Embryo, Nonmammalian / enzymology*
  • Enzyme Inhibitors / pharmacology
  • Indicators and Reagents
  • Kinetics
  • Macromolecular Substances
  • Molecular Weight
  • RNA Nucleotidyltransferases / analysis
  • RNA Nucleotidyltransferases / isolation & purification*
  • RNA Nucleotidyltransferases / metabolism
  • Radioisotope Dilution Technique
  • Tritium

Substances

  • Deoxyadenine Nucleotides
  • Enzyme Inhibitors
  • Indicators and Reagents
  • Macromolecular Substances
  • Tritium
  • Durapatite
  • DNA Primase
  • RNA Nucleotidyltransferases
  • DNA Polymerase II
  • DNA Polymerase III
  • DNA-Directed DNA Polymerase
  • 2'-deoxyadenosine triphosphate