Isoelectric point differentiates PHF-tau from biopsy-derived human brain tau proteins

Neuroreport. 1995 Nov 13;6(16):2217-20. doi: 10.1097/00001756-199511000-00028.


In the present study, Tau proteins were detected by two monoclonal antibodies AD2 and Tau-1 raised against PHF-tau and normal Tau proteins respectively using single- and two-dimensional immunoblotting. We demonstrate here the presence of a Tau triplet in brain homogenates from patients with Alzheimer's disease (AD) processed human brain biopsies from controls. However PHF-tau proteins have a slight but significantly higher mol. wt and a much more acidic isoelectric point. Therefore, Tau proteins are more phosphorylated in AD.

MeSH terms

  • Adult
  • Biopsy
  • Brain Chemistry / physiology*
  • Humans
  • Immunoblotting
  • Isoelectric Point
  • Middle Aged
  • Neurofibrillary Tangles / chemistry*
  • Neurofilament Proteins / chemistry*
  • tau Proteins / chemistry*


  • Neurofilament Proteins
  • tau Proteins