Salmonella Typhimurium acrB-like Gene: Identification and Role in Resistance to Biliary Salts and Detergents and in Murine Infection

FEMS Microbiol Lett. 1996 Jan 15;135(2-3):161-7. doi: 10.1111/j.1574-6968.1996.tb07983.x.

Abstract

Salmonella serotype typhimurium transpositional mutants altered in resistance to biliary salts and detergents were isolated previously. We have characterized further the LX1054 mutant strain, the most sensitive of them. The chromosomal DNA segment flanking transposon insertion was cloned and sequenced. The highest level of identity was found for the acrB (formerly acrE) gene of Escherichia coli, a gene encoding a drug efflux pump of the Acr family. LX1054 exhibited a reduced capacity to colonize the intestinal tract. After passages in mice, the mutant strain lost the sensitive phenotype. In vitro, a resumption of growth appeared after 17 h of culture in medium with cholate or other tested biological or chemical detergents. Then, the acquired resistant phenotype seemed stable. The data suggested a role of S. typhimurium acrB-like gene in resistance to biliary salts and detergents and in mice intestinal colonization. However, the local and transient sensitivity observed in vivo, and the in vitro adaptations suggest that several detergent-resistance mechanisms operate in S. typhimurium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / physiology
  • Base Sequence
  • Carrier Proteins*
  • Cholic Acids / pharmacology*
  • Cloning, Molecular
  • Drug Resistance, Microbial
  • Escherichia coli Proteins*
  • Female
  • Genes, Bacterial*
  • Intestines / microbiology
  • Membrane Proteins / genetics*
  • Membrane Proteins / physiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Multidrug Resistance-Associated Proteins
  • Mutation
  • Salmonella Infections, Animal / microbiology*
  • Salmonella typhimurium / drug effects
  • Salmonella typhimurium / genetics
  • Salmonella typhimurium / pathogenicity
  • Salmonella typhimurium / physiology*
  • Sequence Analysis, DNA
  • Sodium Dodecyl Sulfate / pharmacology

Substances

  • AcrB protein, E coli
  • Bacterial Proteins
  • Carrier Proteins
  • Cholic Acids
  • Escherichia coli Proteins
  • Membrane Proteins
  • Multidrug Resistance-Associated Proteins
  • Sodium Dodecyl Sulfate
  • 3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate

Associated data

  • GENBANK/S81517
  • GENBANK/S81520