Cloning and sequence analysis of the gene encoding 3-hexulose-6-phosphate synthase from the methylotrophic bacterium, Methylomonas aminofaciens 77a, and its expression in Escherichia coli

FEMS Microbiol Lett. 1996 Jan 15;135(2-3):201-5. doi: 10.1111/j.1574-6968.1996.tb07990.x.


A DNA fragment of 550 bp was specifically amplified by PCR with primers based on the N-terminal sequence of the purified 3-hexulose-6-phosphate synthase from Methylomonas aminofaciens 77a and on that of a lysyl endopeptidase-derived peptide. Using this PCR product as a probe, a gene coding for 3-hexulose-6-phosphate synthase in M. aminofaciens 77a chromosomal DNA was cloned in Escherichia coli JM109. Sequencing analysis revealed that the gene encoding 3-hexulose-6-phosphate synthase contained a 624-bp open reading frame, encoding a protein composed of 208 amino acid residues with a calculated relative molecular mass of 21,224.

MeSH terms

  • Aldehyde-Lyases / biosynthesis
  • Aldehyde-Lyases / genetics*
  • Aldehyde-Lyases / isolation & purification
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Genes, Bacterial / genetics*
  • Methylococcaceae / enzymology
  • Methylococcaceae / genetics*
  • Molecular Sequence Data
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / isolation & purification
  • Sequence Alignment
  • Sequence Analysis, DNA


  • Recombinant Proteins
  • Aldehyde-Lyases
  • hexose phosphate synthetase

Associated data

  • GENBANK/D64136