IL-16- and other CD4 ligand-induced migration is dependent upon protein kinase C

Cell Immunol. 1996 Feb 25;168(1):100-6. doi: 10.1006/cimm.1996.0054.


Human interleukin-16, previously known as lymphocyte chemoattractant factor, is a CD4+ T cell competence growth factor initially described as a chemotactic factor for CD4+ cells. The interaction between IL-16 and its receptor CD4 leads to an increase in intracytoplasmic calcium and inositol triphosphate. Because of the association of intracellular shifts in protein kinase C (PKC) enzyme activity with production of these secondary messengers and the participation of PKC in transducing certain receptor-mediated migratory signals, we investigated the role of PKC in the CD4-mediated migratory response by IL-16. Recombinant IL-16 induces rapid translocation of PKC from the cytosol to the membrane in three separate CD4+ cell types: normal blood T cells, SUPT1 cells, and THP1 cells. PKC inhibitors H7, calphostin C, chelerythrine, and bisindolylmaleimide completely block IL-16-induced lymphocyte migration as well as the motile response induced by HIV-1 gp120 and anti-CD4 antibodies. Taken together, these data suggest a role for PKC in CD4-mediated migratory responses.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / metabolism
  • Antibodies, Monoclonal / pharmacology
  • CD4 Antigens / immunology
  • CD4 Antigens / metabolism*
  • CD4 Antigens / pharmacology*
  • Cell Line
  • Cell Movement / drug effects*
  • Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors
  • Enzyme Activation
  • HIV Envelope Protein gp120 / pharmacology
  • Humans
  • Interleukin-16
  • Lymphokines / metabolism
  • Lymphokines / pharmacology*
  • Protein Kinase C / antagonists & inhibitors
  • Protein Kinase C / physiology*
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / enzymology
  • T-Lymphocytes / immunology*


  • Antibodies, Monoclonal
  • CD4 Antigens
  • HIV Envelope Protein gp120
  • Interleukin-16
  • Lymphokines
  • Cyclic AMP-Dependent Protein Kinases
  • Protein Kinase C