Kid, a Novel Kinesin-Like DNA Binding Protein, Is Localized to Chromosomes and the Mitotic Spindle

EMBO J. 1996 Feb 1;15(3):457-67.

Abstract

Microtubule-associated motor proteins are thought to be involved in spindle formation and chromosome movements in mitosis/meiosis. We have molecularly cloned cDNAs for a gene that codes for a novel member of the kinesin family of proteins. Nucleotide sequencing reveals that the predicted gene product is a 73 kDa protein and is related to some extent to the Drosophila node gene product, which is involved in chromosomal segregation during meiosis. A sequence similar to the microtubule binding motor domain of kinesin is present in the N-terminal half of the protein, and its ability to bind to microtubules is demonstrated. Furthermore we show that its C-terminal half contains a putative nuclear localization signal similar to that of Jun and is able to bind to DNA. Accordingly, the protein was termed Kid (kinesin-like DNA binding protein). Indirect immunofluorescence studies show that Kid colocalizes with mitotic chromosomes and that it is enriched in the kinetochore at anaphase. Thus, we propose that Kid might play a role(s) in regulating the chromosomal movement along microtubules during mitosis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Breast Neoplasms / genetics
  • Chromosomes, Human / metabolism
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism*
  • Drosophila / genetics
  • Drosophila Proteins*
  • Female
  • HeLa Cells
  • Humans
  • Kinesin / chemistry
  • Kinesin / genetics*
  • Kinesin / metabolism*
  • Microtubule Proteins / genetics
  • Microtubule-Associated Proteins / chemistry
  • Microtubule-Associated Proteins / genetics*
  • Microtubule-Associated Proteins / metabolism*
  • Mitosis
  • Molecular Sequence Data
  • Molecular Weight
  • Sequence Homology, Amino Acid
  • Spindle Apparatus / metabolism
  • Tumor Cells, Cultured

Substances

  • DNA, Complementary
  • DNA-Binding Proteins
  • Drosophila Proteins
  • KIF22 protein, human
  • Microtubule Proteins
  • Microtubule-Associated Proteins
  • Nod protein, Drosophila
  • Kinesin

Associated data

  • GENBANK/AB017430