A densely methylated DNA island is associated with a chromosomal replication origin in the human RPS14 locus

Somat Cell Mol Genet. 1995 Nov;21(6):369-83. doi: 10.1007/BF02310205.


We describe a 258-bp densely methylated DNA island (DMI) and chromosomal origin of bidirectional DNA replication within the transcribed portion of the human RPS14 intron 1. Together with the DMIs previously detected in two functional Chinese hamster replication origins [see Ref. 1, pp. 5636-5644], observations described in this report strengthen the correlation between densely methylated DNA islands and active mammalian chromosomal replication origins. Accordingly, DMIs may prove to be reliable physical markers for origins of bidirectional DNA replication in complex genomic DNAs of higher animals.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • Cricetinae
  • Cricetulus
  • DNA / chemistry*
  • DNA / genetics*
  • DNA Primers / genetics
  • Genetic Markers
  • Humans
  • Introns
  • Methylation
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Replication Origin / genetics*
  • Sequence Tagged Sites


  • DNA Primers
  • Genetic Markers
  • DNA

Associated data

  • GENBANK/M13934