odr-10 encodes a seven transmembrane domain olfactory receptor required for responses to the odorant diacetyl

Cell. 1996 Mar 22;84(6):899-909. doi: 10.1016/s0092-8674(00)81068-5.


Olfactory signaling is initiated by interactions between odorants and olfactory receptors. We show that the C. elegans odr-10 gene is likely to encode a receptor for the odorant diacetyl. odr-10 mutants have a specific defect in chemotaxis to diacetyl, one of several odorants detected by the AWA olfactory neurons. odr-10 encodes a predicted seven transmembrane domain receptor; a green fluorescent protein-tagged Odr-10 protein is localized to the AWA sensory cilia. odr-10 expression is regulated by odr-7, a transcription factor implicated in AWA sensory specification. Expression of odr-10 from a heterologous promoter directs behavioral responses to diacetyl, but not to another odorant detected by the AWA neurons. These results provide functional evidence for a specific interaction between an olfactory receptor protein and its odorant ligand.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Caenorhabditis elegans / physiology*
  • Chemotaxis / drug effects
  • Chemotaxis / genetics
  • Diacetyl / pharmacology*
  • Gene Expression Regulation / physiology
  • Genes, Helminth / physiology
  • Helminth Proteins / genetics
  • Helminth Proteins / physiology
  • Helminth Proteins / ultrastructure*
  • Male
  • Molecular Sequence Data
  • Mutation
  • Olfactory Receptor Neurons / physiology
  • Olfactory Receptor Neurons / ultrastructure
  • Pyrazines / pharmacology
  • Receptors, Odorant / genetics*
  • Receptors, Odorant / metabolism
  • Receptors, Odorant / ultrastructure
  • Sensitivity and Specificity
  • Signal Transduction / physiology


  • Helminth Proteins
  • Pyrazines
  • Receptors, Odorant
  • Diacetyl

Associated data

  • GENBANK/U49449