Abstract
The effects of the WT on store-mediated Ca2+ entry and protein tyrosine phosphorylation were investigated in fura-2-loaded human platelets. Wortmannin (2 microM) attenuated the rise in [Ca2+]i caused by Ca2+ entry while having no effect on the mobilisation of Ca2+ from internal stores. It also reduced store-depletion-evoked protein tyrosine phosphorylation. These findings demonstrate that WT is an inhibitor of tyrosine phosphorylation and store-mediated calcium entry and provide further evidence for the involvement of a tyrosine phosphorylation step in the link between Ca2+ store depletion and Ca2+ influx in human platelets.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Androstadienes / pharmacology*
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Blood Platelets / drug effects
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Blood Platelets / metabolism*
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Blood Proteins / isolation & purification
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Blood Proteins / metabolism
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Calcium / blood*
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Egtazic Acid / pharmacology
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Electrophoresis, Polyacrylamide Gel
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Enzyme Inhibitors / pharmacology
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Fluorescent Dyes
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Fura-2 / analogs & derivatives
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Humans
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In Vitro Techniques
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Kinetics
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Phosphodiesterase Inhibitors / pharmacology*
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Phosphoproteins / blood*
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Phosphoproteins / isolation & purification
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Phosphorylation
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Phosphotyrosine / analysis
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Terpenes / pharmacology
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Thapsigargin
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Wortmannin
Substances
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Androstadienes
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Blood Proteins
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Enzyme Inhibitors
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Fluorescent Dyes
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Phosphodiesterase Inhibitors
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Phosphoproteins
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Terpenes
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fura-2-am
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Phosphotyrosine
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Egtazic Acid
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Thapsigargin
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Calcium
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Fura-2
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Wortmannin