Identification, cloning and analysis of the Aspergillus niger gene pacC, a wide domain regulatory gene responsive to ambient pH

Mol Gen Genet. 1996 Feb 25;250(3):367-74. doi: 10.1007/BF02174395.


A wide domain regulatory gene implicated in modulating gene expression in response to ambient pH has been cloned and sequenced from the industrially useful filamentous fungus Aspergillus niger. This gene, pacC, is able to restore a pacC+ phenotype to A. nidulans pacCc11 and pacCc14 mutants with respect to extent of conidiation, conidial pigment intensity and acid phosphatase regulation. The pacC gene of A. niger comprises three exons, encodes a three-zinc-finger protein of 677 amino acids, and shows pH-dependent regulation of expression: mRNA levels are elevated under alkaline conditions and considerably reduced under acidic conditions. The occurrence of PacC consensus binding targets within the sequences upstream of pacC may indicate autoregulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acid Phosphatase / metabolism
  • Amino Acid Sequence
  • Aspergillus niger / chemistry
  • Aspergillus niger / genetics*
  • Aspergillus niger / metabolism
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • Electrophoresis, Polyacrylamide Gel
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Gene Expression Regulation, Fungal / genetics
  • Genes, Fungal*
  • Genes, Regulator*
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • Phenotype
  • Protein Biosynthesis / genetics
  • Restriction Mapping
  • Sequence Alignment
  • Transcription Factors / chemistry*
  • Transcription Factors / genetics*
  • Zinc Fingers / genetics


  • Fungal Proteins
  • PacC protein, Aspergillus
  • Transcription Factors
  • Acid Phosphatase

Associated data

  • GENBANK/X98417