Circadian rhythms in cultured mammalian retina

Science. 1996 Apr 19;272(5260):419-21. doi: 10.1126/science.272.5260.419.


Many retinal functions are circadian, but in most instances the location of the clock that drives the rhythm is not known. Cultured neural retinas of the golden hamster (Mesocricetus auratus) exhibited circadian rhythms of melatonin synthesis for at least 5 days at 27 degrees celsius. The rhythms were entrained by light cycles applied in vitro and were free-running in constant darkness. Retinas from hamsters homozygous for the circadian mutation tau, which shortens the free-running period of the circadian activity rhythm by 4 hours, showed a shortened free-running period of melatonin synthesis. The mammalian retina contains a genetically programmed circadian oscillator that regulates its synthesis of melatonin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biological Clocks*
  • Circadian Rhythm* / genetics
  • Cricetinae
  • Culture Techniques
  • Darkness
  • Genes
  • Light
  • Melatonin / biosynthesis*
  • Mesocricetus
  • Mutation
  • Retina / metabolism
  • Retina / physiology*
  • Temperature


  • Melatonin