Response of Drosophila metallothionein promoters to metallic, heat shock and oxidative stresses

FEBS Lett. 1996 Feb 12;380(1-2):33-8. doi: 10.1016/0014-5793(95)01544-2.

Abstract

The metallothionein system in Drosophila melanogaster is composed of two genes, Mtn and Mto. In order to compare the induction properties of these genes, we transformed D. melanogaster with P-element vectors containing Adh and lacZ reporter genes under the control of Mtn and Mto promoters, respectively. Mtn and Mto transgenes are mainly expressed in digestive tract. However, Mtn expression has been detected also in the fat body. Mtn and Mto transgenes respond differently to metallic, heat-shock and oxidative stresses. These data confirm that both genes are in part functionally different.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alcohol Dehydrogenase / genetics
  • Animals
  • Digestive System / growth & development
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / growth & development
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / physiology
  • Genes, Insect
  • Genes, Reporter
  • Heat-Shock Response / genetics*
  • Homeostasis / genetics
  • Larva
  • Metallothionein / genetics*
  • Metals / pharmacology*
  • Oxidative Stress / genetics*
  • Paraquat / pharmacology
  • Promoter Regions, Genetic / physiology*
  • Transgenes
  • beta-Galactosidase / genetics

Substances

  • Metals
  • Metallothionein
  • Alcohol Dehydrogenase
  • beta-Galactosidase
  • Paraquat