Intraocular levels of methotrexate after intravenous administration

Am J Ophthalmol. 1996 Apr;121(4):442-4. doi: 10.1016/s0002-9394(14)70444-1.


Purpose: To determine if adequate intraocular levels of methotrexate are achieved after intravenous administration.

Methods: After intravenous administration, methotrexate levels were determined in the serum, the anterior chamber, and the cerebrospinal fluids of a patient with recurrent ocular lymphoma. A fluorescence polarization immunoassay was used to make the determinations.

Results: At seven hours into a 24-hour intravenous infusion, methotrexate was at cytotoxic level in all samples. At 74 hours, cytotoxic levels were present only in the aqueous humor.

Conclusion: Sustained cytotoxic ocular methotrexate levels are achievable after systemic administration.

Publication types

  • Case Reports

MeSH terms

  • Anterior Chamber / metabolism
  • Antimetabolites, Antineoplastic / administration & dosage
  • Antimetabolites, Antineoplastic / pharmacokinetics*
  • Antimetabolites, Antineoplastic / therapeutic use
  • Aqueous Humor / metabolism*
  • Biological Availability
  • Cerebrospinal Fluid / metabolism
  • Eye Neoplasms / drug therapy
  • Eye Neoplasms / metabolism
  • Female
  • Fluorescence Polarization Immunoassay
  • Humans
  • Infusions, Intravenous
  • Lymphoma, Non-Hodgkin / drug therapy
  • Lymphoma, Non-Hodgkin / metabolism
  • Methotrexate / administration & dosage
  • Methotrexate / pharmacokinetics*
  • Methotrexate / therapeutic use
  • Middle Aged


  • Antimetabolites, Antineoplastic
  • Methotrexate