Abstract
Neutrophil migration and activation are the cornerstones of the acute inflammatory response. Interleukin-8 triggers several functions of neutrophils in host defense: chemotaxis, degranulation and enzyme release, and superoxide production. Interleukin-8 is most potent as a chemoattractant, so chemotaxis is likely the most important of these functions. The effects of interleukin-8 on neutrophils are mediated through two receptors, IL-8RA and IL-8RB. To investigate the role of these receptors in neutrophil chemotaxis, we produced inhibitory antibodies to IL-8RA. These antibodies inhibit neutrophil chemotaxis toward IL-8 in vitro. These findings show that IL-8RA mediates a chemotactic signal in neutrophils and suggest that an anti-receptor strategy may be a useful approach to limit neutrophil migration in inflammation.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies / pharmacology
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Antibodies, Monoclonal / pharmacology
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Antigens, CD / biosynthesis
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Antigens, CD / immunology
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Antigens, CD / physiology*
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Calcium / metabolism
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Chemotaxis, Leukocyte* / drug effects
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Complement C5a / pharmacology
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Epitopes / analysis
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Immunoglobulin Fab Fragments
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Immunoglobulin G
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Interleukin-8 / pharmacology*
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Mice
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Mice, Inbred BALB C
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N-Formylmethionine Leucyl-Phenylalanine / pharmacology
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Neutrophils / physiology*
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Polymerase Chain Reaction
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Rabbits
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Receptors, Interleukin / biosynthesis
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Receptors, Interleukin / immunology
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Receptors, Interleukin / physiology*
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Receptors, Interleukin-8A
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Receptors, Interleukin-8B
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / immunology
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Transfection
Substances
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Antibodies
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Antibodies, Monoclonal
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Antigens, CD
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Epitopes
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Immunoglobulin Fab Fragments
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Immunoglobulin G
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Interleukin-8
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Receptors, Interleukin
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Receptors, Interleukin-8A
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Receptors, Interleukin-8B
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Recombinant Proteins
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N-Formylmethionine Leucyl-Phenylalanine
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Complement C5a
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Calcium