In vivo effects of tumor necrosis factor-alpha on incised wound and gunshot wound healing

J Trauma. 1996 Mar;40(3 Suppl):S140-3. doi: 10.1097/00005373-199603001-00030.


Recombinant tumor necrosis factor alpha (TNF-alpha) was applied to incised wounds (adult rats, n = 14) and gunshot wounds (dogs, n = 5). The accumulation of RNA and DNA in regenerative tissue, the wound disruption strength (WDS), and histology were examined. The results showed that a single dose of TNF-alpha in saline with highly purified bovine serum albumin increased the WDS and accumulation of RNA and DNA in regenerative tissue in incised wounds 3 days after surgery (p < 0.05). Also, TNF-alpha promoted the inflammatory reaction in the wounded area of gunshot wounds. Histology revealed increased capillary tube-like structures and collagen formation and fibroblast proliferation in both incised and gunshot wounds treated with TNF-alpha. The results indicated that locally applied TNF-alpha benefited the incised and gunshot wound healing. These mitogenic and inflammatory effects of TNF-alpha may be the synergistic effects with other growth factors such as platelet-derived growth factor (PDGF).

MeSH terms

  • Animals
  • DNA / biosynthesis
  • Dogs
  • Male
  • RNA / biosynthesis
  • Rats
  • Recombinant Proteins / pharmacology
  • Tumor Necrosis Factor-alpha / pharmacology*
  • Wound Healing / drug effects*
  • Wounds, Gunshot / drug therapy
  • Wounds, Gunshot / pathology
  • Wounds, Gunshot / physiopathology*
  • Wounds, Stab / drug therapy
  • Wounds, Stab / pathology
  • Wounds, Stab / physiopathology*


  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • RNA
  • DNA