This article describes nerve conduction studies of the deep temporal nerve (DTN) and the mylohyoid nerve (MHN) motor branches of the trigeminal nerve. These nerves were stimulated intraorally with a pediatric surface stimulator. Compound muscle action potentials were recorded over the temporalis and mylohyoid muscles with surface electrodes. Forty-two subjects were studied. In all subjects the MHN response was elicited bilaterally, giving an upper latency limit of 2.3 ms. The mean MHN amplitude was 4.9 mV (SD = 1.8 mV, minimum = 1.3 mV). The maximal side-to-side amplitude difference was 0.4 ms. and the maximal side-to-side amplitude difference was 2.2 mV. The DTN response was only elicited bilaterally in 25 (60%) subjects. The average DTN latency was 2.1 ms (SD = 0.3, maximum = 2.7 ms). The average DTN amplitude was 4.3 mV (SD = 2.0, minimum = 0.3 mV). The MHN responses were the least technically demanding, and were more consistently elicited than the DTN responses. These nerve conduction techniques should prove useful in patients with trigeminal nerve disorders.