Molecular cloning and characterization of a cDNA encoding a novel basic helix-loop-helix protein structurally related to Neuro-D/BHF1

Biochem Biophys Res Commun. 1996 Mar 27;220(3):754-8. doi: 10.1006/bbrc.1996.0476.

Abstract

We cloned a novel basic helix-loop-helix protein, NDRF (NeuroD-related factor), cDNA. NDRF contains 383 amino acids and exhibits 53.4% and 52.2% sequence identity to NeuroD and MATH-2/NEX-1, respectively. NDRF mRNA appears in the brain of 12-day-old mouse embryos and is localized in certain regions of the adult brain, such as the hippocampus, dentate gyrus and cerebellum, Thus, the structure and expression patterns of NDRF are similar to but distinct from those of NeuroD and MATH-2/NEX-1, suggesting that NDRF may play distinct roles in neural development and plasticity as a novel member of the NeuroD family.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • Brain / cytology
  • Brain / metabolism*
  • Cloning, Molecular
  • Conserved Sequence
  • DNA, Complementary
  • Embryo, Mammalian
  • Gene Expression
  • Gene Library
  • Helix-Loop-Helix Motifs*
  • In Situ Hybridization
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry*
  • Neurons / cytology
  • Neurons / metabolism
  • Neuropeptides / biosynthesis*
  • Neuropeptides / chemistry*
  • Organ Specificity
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • Rats
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Transcription Factors / chemistry*

Substances

  • Basic Helix-Loop-Helix Transcription Factors
  • DNA, Complementary
  • Nerve Tissue Proteins
  • Neurod2 protein, mouse
  • Neurod2 protein, rat
  • Neurod6 protein, mouse
  • Neurod6 protein, rat
  • Neuropeptides
  • RNA, Messenger
  • Transcription Factors
  • Neurogenic differentiation factor 1

Associated data

  • GENBANK/D83507