Novel Specific Chemtactic Receptor for S100L Protein on Guinea Pig Eosinophils

Biochem Biophys Res Commun. 1996 Mar 27;220(3):871-4. doi: 10.1006/bbrc.1996.0496.

Abstract

We isolated a new chemotactic protein from bovine lung, and its partial protein sequence analysis indicated that this protein was identical with S100L, one member of the Ca2+-binding S100 protein family. The chemotactic activity of S100L on guinea pig eosinophils is observed at 0.1nM protein concentration, and the effects appear mediated by a novel specific surface receptor. We characterized the receptor for S100L on guinea pig eosinophils. Scatchard analyses of the data that [125I]S100L bound to S100L receptor indicate the presence of two receptor populations on eosinophils with a Kd value of 3.3 x 10(-11)M and 1.1 x 10(-9)M. Thus, S100L protein has the most potent chemotactic activity on guinea pig eosinophils of all the chemotactic proteins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / metabolism
  • Cattle
  • Chemotactic Factors / isolation & purification
  • Chemotactic Factors / metabolism*
  • Chemotactic Factors / pharmacology*
  • Chemotaxis, Leukocyte* / drug effects
  • Eosinophils / drug effects
  • Eosinophils / physiology*
  • Guinea Pigs
  • Kinetics
  • Lung / metabolism
  • Myocardium / metabolism
  • Receptors, Formyl Peptide
  • Receptors, Immunologic / drug effects
  • Receptors, Immunologic / metabolism
  • Receptors, Immunologic / physiology*
  • Receptors, Peptide / drug effects
  • Receptors, Peptide / metabolism
  • Receptors, Peptide / physiology*
  • S100 Proteins / isolation & purification
  • S100 Proteins / metabolism*
  • S100 Proteins / pharmacology*
  • Substrate Specificity
  • Swine
  • Virulence Factors, Bordetella / pharmacology

Substances

  • Chemotactic Factors
  • Receptors, Formyl Peptide
  • Receptors, Immunologic
  • Receptors, Peptide
  • S100 Proteins
  • S100A2 protein, human
  • Virulence Factors, Bordetella