Cytokine-induced neutrophil chemoattractant (CINC)-2 alpha, a novel member of rat GRO/CINCs, is a predominant chemokine produced by lipopolysaccharide-stimulated rat macrophages in culture

Biochem Biophys Res Commun. 1996 Mar 27;220(3):945-8. doi: 10.1006/bbrc.1996.0511.


Neutrophil chemotactic factors (chemokines) have been purified from conditioned medium of lipopolysaccharide (LPS)-stimulated rat macrophages in culture. The LPS-stimulated macrophages produced one acidic chemokine, rat macrophage inflammatory protein (MIP)-1alpha, and four basic chemokines, cytokine-induced neutrophil chemoattractant (CINC)-1. CINC-2alpha, CINC-2beta and CINC-3/rat MIP-2. CINC-2alpha, a novel chemokine recently isolated from conditioned medium of rat granulation-tissue culture, was the major chemoattractant among these four basic chemokines. The results suggest that CINC-2alpha is produced by activated macrophages in vivo and plays an important role in the infiltration of neutrophils into inflammatory sites in rats

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cells, Cultured
  • Chemokine CXCL1
  • Chemokine CXCL2
  • Chemokines / biosynthesis*
  • Chemokines / chemistry
  • Chemokines, CXC*
  • Chemotactic Factors / biosynthesis*
  • Chemotactic Factors / chemistry
  • Chemotactic Factors / pharmacology
  • Chemotaxis, Leukocyte
  • Culture Media, Conditioned
  • Growth Substances / biosynthesis*
  • Growth Substances / chemistry
  • Growth Substances / pharmacology
  • Humans
  • Intercellular Signaling Peptides and Proteins*
  • Lipopolysaccharides / pharmacology*
  • Macrophages, Peritoneal / drug effects
  • Macrophages, Peritoneal / metabolism*
  • Male
  • Molecular Sequence Data
  • Neutrophils / drug effects
  • Neutrophils / physiology
  • Rats
  • Rats, Wistar
  • Sequence Homology, Amino Acid


  • CXCL1 protein, human
  • Chemokine CXCL1
  • Chemokine CXCL2
  • Chemokines
  • Chemokines, CXC
  • Chemotactic Factors
  • Culture Media, Conditioned
  • Cxcl1 protein, rat
  • Cxcl2 protein, rat
  • Cxcl3 protein, rat
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Lipopolysaccharides