Identification of a Stat Gene That Functions in Drosophila Development

Cell. 1996 Feb 9;84(3):421-30. doi: 10.1016/s0092-8674(00)81287-8.

Abstract

A Drosophila Stat gene (D-Stat) with a zygotic segmental expression pattern was identified. This protein becomes phosphorylated on Tyr-704 when coexpressed in Schneider cells with a Drosophila janus kinase (JAK), Hopscotch (HOP). The phosphorylated protein binds specifically to the consensus sequence TTCCCGGAA. Suppressor mutations of hopTum-I, a dominant hyperactive allele of hop whose phenotype is hematocyte overproduction and tumor formation, were selected. One of these mutants, statHJ, mapped to the same chromosomal region (92E) as does D-Stat, had an incompletely penetrant pair rule phenotype, and exhibited aberrant expression of the pair rule gene even skipped (eve) at the cellular blastoderm stage. Two D-STAT-binding sites were identified within the eve stripe 3 enhancer region. Mutations in either of the STAT-binding sites greatly decreased the stripe 3 expression in transgenic flies. Clearly, the JAK-STAT pathway is connected to Drosophila early development.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites / genetics
  • Chromosome Mapping
  • Consensus Sequence
  • DNA / genetics
  • DNA / metabolism
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Drosophila / embryology*
  • Drosophila / genetics*
  • Drosophila / metabolism
  • Drosophila Proteins
  • Gene Expression Regulation, Developmental
  • Genes, Insect*
  • Janus Kinases
  • Molecular Sequence Data
  • Phosphorylation
  • Promoter Regions, Genetic
  • Protein-Tyrosine Kinases / genetics
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Trans-Activators / chemistry
  • Trans-Activators / genetics*
  • Trans-Activators / metabolism
  • Transcription Factors

Substances

  • DNA-Binding Proteins
  • Drosophila Proteins
  • Trans-Activators
  • Transcription Factors
  • DNA
  • Protein-Tyrosine Kinases
  • Janus Kinases
  • hop protein, Drosophila

Associated data

  • GENBANK/U46688