Induction of axial mesoderm by zDVR-1, the zebrafish orthologue of Xenopus Vg1

Dev Biol. 1996 Apr 10;175(1):108-17. doi: 10.1006/dbio.1996.0099.


The zebrafish DVR-1 (zDVR-1) gene, like Xenopus Vg1, is present maternally as an unprocessed precursor protein which is distributed ubiquitously along the future dorsoventral axis. Also, like Vg1, overexpression of zDVR-1 in zebrafish directs synthesis of more precursor, but no processed protein. However, the native zDVR-1 precursor is processed to mature protein when expressed in Xenopus. Like processed Vg1, mature zDVR-1 is a potent inducer of axial mesoderm. The parallels in expression pattern, apparent regulation of protein processing, and mesoderm-inducing activity support the hypothesis that localized protein processing controls production of a dorsal mesoderm inducer in these two species. Furthermore, using mutant mRNAs, we show that cleavage site sequences of the precursor protein are important in regulating protein processing.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA-Binding Proteins / biosynthesis
  • Embryonic Induction*
  • Gene Expression Regulation, Developmental
  • Glycoproteins / metabolism*
  • Goosecoid Protein
  • Homeodomain Proteins*
  • Mesoderm / physiology*
  • Molecular Sequence Data
  • Protein Precursors / metabolism
  • Protein Processing, Post-Translational
  • Repressor Proteins*
  • Species Specificity
  • Tissue Distribution
  • Transcription Factors*
  • Transforming Growth Factor beta / metabolism*
  • Xenopus
  • Xenopus Proteins
  • Zebrafish / embryology*
  • Zebrafish Proteins


  • DNA-Binding Proteins
  • GDF1 protein, Xenopus
  • Gdf3 protein, zebrafish
  • Glycoproteins
  • Goosecoid Protein
  • Homeodomain Proteins
  • Protein Precursors
  • Repressor Proteins
  • Transcription Factors
  • Transforming Growth Factor beta
  • Xenopus Proteins
  • Zebrafish Proteins
  • gsc protein, zebrafish